Thistle Pollen


coker

Link Posted 10/07/2019 - 17:31
K5, SMC Pentax-M 200mm f4 + SMC Pentax-M 50mm f1.7 reversed.
AF280FT on camera diffused flash.
Manual focus & exposure.
39 frames, in 3 slabs, stacked in Combine ZP.
Elements 11.








Any thoughts or comments most welcome, thanks for looking,

Roger.
The more I look, the more there is to see!

Chrism8

Link Posted 10/07/2019 - 18:18
The second image is particularly good 😁
Chris

www.chrismillsphotography.co.uk

" A Hangover is something that occupies the Head you neglected to use the night before".

-------------------------------------------------------------
K1 - Sigma 85mm F1.4, Pentax 150 -450 F4.5 / 5.6, Pentax FA 24 - 70 F2.8

Sigma 100-300 F4, Samyang 14mm F2.8, Sigma 70-200 F2.8,

K5iis - Sigma 17 - 70 F2.8, Sigma 70 - 300 F3.5/F5.6, Sigma 18 - 200 F3.6 / F4.5.

Lubbyman

Link Posted 10/07/2019 - 19:28
Phenomenal!

Have you any idea what the diameter of one of those pollen grains is?

Steve

derek897

Link Posted 10/07/2019 - 22:51
Really nice images. Really good display of what a good focus stacking workflow can achieve.
Hats off to you sir
I know what i like, If not always why.

coker

Link Posted 10/07/2019 - 22:53
Thanks,Chaps.

Put an uncropped frame of the stack on my screen.
(Roughly) measured the frame width on screen as 230mm, & one of the grains ,on the same screen, as 2mm.
The grain is, therfore approx. 1/115th. of the (APSC) frame width.
APSC frame with is approx 23.7mm.
23.7mm divided by 115 = roughly 0.2.

The grains are approx,. 0.2mm in diameter.

Is this ok, does it work, is it about right..............?

(Edit)
No.This combination of lenses gives a repro. ratio of 4:1, so.......
Does that make the grain (0.2 divided by 4) = 0.05mm.
Excuse me, my brain hurts.
I'm going to bed......

Thanks for looking,

Roger.
The more I look, the more there is to see!
Last Edited by coker on 10/07/2019 - 23:05

HarisF1

Link Posted 10/07/2019 - 23:22
That's just stunning work, well done. Love seeing stuff like this (that's genuine backyard science!)

pschlute

Link Posted 10/07/2019 - 23:55
I really like the second one too.

+1 agreeing with the backyard science comment above:

2 lenses (one reversed)

a flash

39 frames

3 slabs (whatever they are)

great work.
Peter



My Flickr page
Last Edited by pschlute on 10/07/2019 - 23:55

Lubbyman

Link Posted 11/07/2019 - 13:57
coker wrote:
Does that make the grain (0.2 divided by 4) = 0.05mm.

Wikipedia says the diameter of a pollen grain is between 0.006mm and 0.1mm, depending on species. So your estimate looks pretty good to me. And you've got detail on those grains that could well be 1/10 of 0.05mm = 0.005mm.

Have you ever thought about getting a scanning electron microscope? No need for focus stacking, think of the time you'll save!!

Steve

Sry

Link Posted 11/07/2019 - 21:50
Impressiv!e! Composition-wise, I prefer the second image - very well processed, too

coker

Link Posted 11/07/2019 - 22:56
After a tentative enquiry, shall we just say it would have to be birthday & Christmas combined,

OR

the basement gym & cinema would have to be put on hold

AND

the shepherd's hut & the beach hut at Sandbanks would have to go as the down-payment......

Oh, sorry!


It seems I've been dream-posting.....

Roger.

The more I look, the more there is to see!

coker

Link Posted 11/07/2019 - 23:02
Peter, a slab is a sub-stack.
So, in this case 3 slabs x 13 as opposed to 1 stack x 39 frames.
I find it's a bit quicker.

( NB but see above,,,)

Roger.
The more I look, the more there is to see!
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